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RNA

Part:BBa_K2591022:Design

Designed by: Luo Kevin   Group: iGEM18_SUSTech_Shenzhen   (2018-10-11)


A gRNA designed to Wnt3A, an essential gene to secretion of the canonical wnt protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

in our part, we design different gRNAs to knockout the gene which function the secretion of the Wnt3A protein, besides, we also design a gRNA to the Wnt3A gene as a positive control group to ensure the normal function of each gRNA.


Source

the sequence of gRNA is from an exon of the mouse Wnt3A gene.

References